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Ultra fast analysis of subcutaneous pork fat

Author:
Ficarra, Alessandro, Fiego, Domenico Pietro Lo, Minelli, Giovanna, Antonelli, Andrea
Source:
Food chemistry 2010 v.121 no.3 pp. 809-814
ISSN:
0308-8146
Subject:
pork, subcutaneous fat, food analysis, rapid methods, meat composition, carcass composition, catalysts, methylation, analytical methods, transesterification, fatty acid composition, fatty acids, triacylglycerols
Abstract:
Narrow-bore GC columns have led to the resolution of complex matrices in a few minutes. To set up a method able to cope with the velocity of ultra fast gas-chromatographic separation, a comparison among four of the most popular lipid transmethylation was applied to pork fat. Different catalysts for transmethylation were tested (NaOCH₃, BF₃, H₂SO₄, and KOH), and were compared for yield and rate of transmethylation, cost, and safety. In all cases, KOH gave the best performance of all the considered parameters. For this reason, it is particularly suitable when a large number of samples are being analysed. Although it is very effective in the transesterification of neutral lipids, it does not tolerate water and is unsuitable for free fatty acid derivatization.
Agid:
779433