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Effects of cytokinins, carbohydrates and amino acids on induction and maturation of somatic embryos in kodo millet (Paspalum scorbiculatum Linn.)

Author:
Ceasar, S. Antony, Ignacimuthu, S.
Source:
Plant cell, tissue, and organ culture 2010 v.102 no.2 pp. 153-162
ISSN:
0167-6857
Subject:
Paspalum scrobiculatum, somatic embryogenesis, plantlets, rooting, culture media, kinetin, 2,4-D, maltose, proline, in vitro regeneration, micropropagation
Abstract:
The effects of cytokinins, carbohydrates and amino acids were studied on maturation and regeneration of embryogenic callus (EC) in kodo millet (Paspalum scorbiculatum Linn.) using a two-step culture procedure. The highest percentage (87.6%) of EC induction was obtained in the induction medium containing Murashige and Skoog (MS) basal salts supplemented with 9.0 μM 2,4-dichlorophenoxy acetic acid and 2.25 μM kinetin. This EC was subcultured in the maturation medium for somatic embryo (SE) maturation and plantlet formation. Addition of cytokinins, carbohydrates and amino acids in the maturation medium promoted the SE maturation and plantlet formation. The maturation medium containing MS basal salts amended with 4.50 μM thidiazuron, 120 mM maltose and 200 μM l-proline gave the maximum number of SEs (39.4) and plantlets (31.3). Plantlets were successfully grown to maturity after hardening in the soil.
Agid:
787759