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Regeneration of daylily (Hemerocallis) from young leaf segments

Li, Zhiwu, Mize, Katherine, Campbell, Faith
Plant cell, tissue, and organ culture 2010 v.102 no.2 pp. 199-204
Hemerocallis, ornamental plants, nursery crops, micropropagation, leaves, explants, callus culture, callus formation, culture media, 2,4-D, benzyladenine, plant growth substances, application rate, naphthaleneacetic acid, adventitious shoots, light, rooting, acclimation
Calli were initiated from leaf segments (~0.5 × 0.5 cm) of daylily incubated on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-dichlorophenoxyacetic acid (2.4-D), and either benzyladenine (BA) or thidiazuron (TDZ). The highest frequency of callus induction was observed on medium with 6.79 μM 2,4-D plus either 4.55 or 6.81 μm TDZ. A period of callus maintenance on medium containing 5.37 μM naphthaleneacetic acid (NAA) plus 2.22 or 4.44 μM BA was necessary following induction to improve the quality of the callus, and significantly increase the frequency of embryogenic-like callus formation and shoot regeneration once calli were transferred to light. Over 70% of the regenerated shoots produced roots on ½ strength MS medium lacking plant growth regulators. The regenerated plantlets were successfully transferred into soil and acclimatized in growth rooms. This is the first report showing that leaf segments can be used for daylily regeneration.