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Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus
- Wang, Maohua, Liu, Mianxue, Li, Dekuan, Wu, Jun, Li, Xufeng, Yang, Yi
- Plant cell, tissue, and organ culture 2010 v.102 no.2 pp. 205-211
- Brassica napus, rapeseed products, oil crops, seed germination, hypocotyls, gene expression, stearoyl-CoA desaturase, oleic acid, linoleic acid, lipid metabolism, plant development, gene expression regulation, promoter regions, messenger RNA, transgenic plants
- The enzyme fatty acid desaturase 2 (FAD2) transforms oleic acid (C18:1) to linoleic acid (C18:2) in plants and as such is involved in fatty acid synthesis. It is also involved in plant development and self-defense, such as seed germination, leaf expansion and cold resistance. We have cloned the full coding region of the Brassica napus FAD2 gene and ectopically expressed it in B. napus expressing low levels of FAD2. Overexpression of FAD2 under the control of the CaMV 35S promoter resulted in an up-regulated FAD2 mRNA level in B. napus as expected. Further analysis revealed that the FAD2 transgenic lines varied greatly in terms of their physiological characteristics, such as enhanced seed germination and increased hypocotyl length, compared to non-transgenic plants, suggesting that up-regulated FAD2 can promote seed germination and hypocotyl elongation in B. napus. Our results demonstrate the possible roles of FAD2 in plant development and also provide a platform for further analysis of fatty acid synthesis in plants.