Main content area

Molecular cloning and characterization of equine thymic stromal lymphopoietin

Klukowska-Rötzler, J., Marti, E., Bugno, M., Leeb, T., Janda, J.
Veterinary immunology and immunopathology 2010 v.136 no.3-4 pp. 346-349
horses, interleukin-7, molecular genetics, genome, genomics, genes, molecular cloning, complementary DNA, exons, phylogeny, immune response, inflammation, T-lymphocytes, lymphocyte proliferation, messenger RNA, gene expression, hypersensitivity, asthma, dermatitis
Thymic stromal lymphopoietin (TSLP) is a novel cytokine that plays a central role in T helper 2 (Th2) cell differentiation and allergic inflammation. It is predominantly expressed by epithelial cells, and its expression is increased in patients with atopic dermatitis and asthma. Mice overexpressing TSLP in the skin develop allergic dermatitis and mice overexpressing TSLP in lungs develop asthma-like disease. However, it is not known whether TSLP plays an important role in equine allergies. Therefore, we cloned and sequenced the complete translated region of equine TSLP gene and measured its expression in various tissues. The equine TSLP gene is organized in 4 exons and encodes a protein of 143 amino acids, which has 62% amino acid identity with human TSLP.