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Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium

Todorov, Svetoslav Dimitrov, Wachsman, Monica, Tomé, Elisabetta, Dousset, Xavier, Destro, Maria Teresa, Dicks, Leon Milner Theodore, Franco, Bernadette Dora Gombossy de Melo, Vaz-Velho, Manuella, Drider, Djamel
Food microbiology 2010 v.27 no.7 pp. 869-879
antiviral properties, pediocins, bacteriocins, Enterococcus faecium, smoked fish, salmon, food pathogens, antibacterial properties, food contamination
The bacteriocin-producing strain Enterococcus faecium ST5Ha was isolated from smoked salmon and identified by biomolecular techniques. Ent. faecium ST5Ha produces a pediocin-like bacteriocin with activity against several lactic acid bacteria, Listeria spp. and some other human and food pathogens, and remarkably against HSV-1 virus. Bacteriocin ST5Ha was produced at high levels in MRS broth at 30 °C and 37 °C, reaching a maximum production of 1.0 × 109 AU/ml, checked against Listeria ivanovii ATCC19119 as target strain and surrogate of pathogenic strain Listeria monocytogenes. The molecular weight of bacteriocin ST5Ha was estimated to be 4.5 kDa according to tricine-SDS-PAGE data. Ent. faecium ST5Ha harbors a 1.044 kb chromosomal DNA fragment fitting in size to that of pediocin PA-1/AcH. In addition, the sequencing of bacteriocin ST5Ha gene indicated 99% of DNA homology to pediocin PA-1/AcH. The combined application of low levels (below MIC) of ciprofloxacin and bacteriocin ST5Ha resulted in a synergetic effect in the inhibition of target strain L. ivanovii ATCC19119. Bacteriocin ST5Ha displayed antiviral activity against HSV-1, an important human pathogen, with a selectivity index of 173. To the best of our knowledge, this is the first report on Ent. faecium as a potential producer of pediocin-like bacteriocin with antiviral activity.