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Separation and Detection of Cell Wall-Bound Ferulic Acid Dehydrodimers and Dehydrotrimers in Cereals and Other Plant Materials by Reversed Phase High-Performance Liquid Chromatography With Ultraviolet Detection

Dobberstein, Diane, Bunzel, Mirko
Journal of agricultural and food chemistry 2010 v.58 no.16 pp. 8927-8935
grains, vegetables, cell wall components, ferulic acid, analytical methods, detection, fractionation, reversed-phase high performance liquid chromatography, chemical constituents of plants, biopolymers, calibration, validity, lignin, precision, accuracy, mass spectrometry, corn, wheat, rye, wild rice, asparagus spears, sugar beet, quantitative analysis, qualitative analysis
Ferulate dehydrodimers and the more recently discovered dehydrotrimers play an important role in the cell wall architecture of plant-based foods and forages. High-performance liquid chromatography methods to determine ferulate dimers often lack specificity; methods for trimers did not exist yet. A method for the determination of 11 cell wall-bound ferulate dehydrodimers and -trimers was developed, including the crucial separation of the di/trimers from the often dominating phenolic monomers. Validation parameters for the basic calibration of the dimers and trimers met our acceptance criteria. However, the matrix calibration revealed that lignin-rich matrices lead to problems with precision and accuracy that likely can be addressed by using a more specific detection, that is, mass spectrometric detection, next to improved sample preparation procedures. The method was used to analyze low-lignin fibers from corn, wheat, and rye grains, wild rice, asparagus, and sugar beet. With the exception of wild rice, the 5-5/8-O-4-, 8-O-4/8-O-4-, and 8-8(aryltetralin)/8-O-4-dehydrotrimers were detected in all analyzed samples, however, often in amounts below the limit of quantitation.