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Analysis of residual monepantel sulfone in milk using icELISA based on a monoclonal antibody developed from an easy-synthesized and low-cost hapten

Yanfang Zhang, Pimiao Zheng, Zile Wang, Zhuoren Ling, Zhenhui Ren, Haiyang Jiang
Journal of food composition and analysis 2023 v.115 pp. 104897
antiparasitic agents, detection limit, enzyme-linked immunosorbent assay, food composition, haptens, immunomagnetic separation, milk, monoclonal antibodies
Monepantel (MNP) is a new class of aminoacetonitrile derivative antiparasitic drug and mainly metabolizes as monepantel sulfone in vivo. This study aimed at developing a novel monepantel sulfone (MNPS) detection method combining indirect competitive enzyme-linked immunosorbent assay (icELISA) and immunomagnetic separation. An easy-synthesized hapten was designed based on the partial structure of MNPS. A monoclonal antibody was obtained by immunizing mice, and applied to icELISA. The half inhibition concentration (IC₅₀) was 1.36 ng/mL, the limit of detection (LOD) was 0.25 μg/L. The average recoveries of the icELISA ranged from 89.5% to 114.5% with the coefficient of variation lower than 13.2%. In summary, a rapid and reliable icELISA was developed for detecting MNPS in milk samples for the first time.