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Oral plasma proteins attenuate gut inflammatory effects induced by S. aureus enterotoxin B challenge in rats

Pérez-Bosque, Anna, Miró, Lluïsa, Polo, Javier, Russell, Louis, Campbell, Joy, Weaver, Eric, Crenshaw, Joe, Moretó, Miquel
Livestock science 2010 v.133 no.1-3 pp. 242-245
Staphylococcus aureus, anti-inflammatory activity, lymphatic system, intestinal mucosa, leukotrienes, oral administration, interferons, bacterial toxins, spray drying, interleukin-10, blood plasma, feed supplements, enterotoxins, temporal variation, nitric oxide synthase, polymerase chain reaction, tumor necrosis factor-alpha, rats, protein synthesis, inflammation, interleukin-6, animal models
In previous studies we have shown that dietary supplementation with spray-dried porcine plasma proteins (SDP) attenuates the effects of the S. aureus enterotoxin B (SEB) on activation of gut-associated lymphoid tissue (GALT) and increased intestinal permeability. We have now studied if the effects of plasma supplementation correlated with mucosal cytokine release and defensin expression. Wistar-Lewis rats were fed diets supplemented with SDP (8% w/w), with a plasma protein fraction containing 50% Ig (PPF; 1.5% w/w) or with milk proteins (Control diet), Animals were fed experimental diets from weaning (day 21) until day 35 after birth, and on days 32 and 35, were administered SEB (i.p. 0.5mg/kg) to induce a mild intestinal inflammation. Experimental groups were Control, SEB, SEB-SDP and SEB-PPF. Cytokines were determined by a cytometric bead array assay, LTB₄ by ELISA and iNOS and defensins by real time PCR, all at 6h after SEB administration. Results showed that SEB significantly increased mucosal IFN-γ, TNF-α, IL-6 and LTB₄ concentration (by 110%, 20%, 30% and 110%, respectively; all P<0.05). SDP and PPF partially attenuated these effects. SEB increased mucosal iNOS expression by 22% (P<0.05) and both plasma protein supplements prevented SEB effects on iNOS expression (both P<0.05). SEB administration also reduced the expression of β-defensin 1 and cryptdin 4 about 10% (P<0.05) and both supplements significantly prevented these effects (P<0.05). SDP increased the expression of mucosal IL-10 supporting the view that this anti-inflammatory cytokine participates in the mechanism of action of dietary plasma proteins during intestinal inflammation.