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LC−MS Determination and Pharmacokinetics of p-Coumaric Acid in Rat Plasma after Oral Administration of p-Coumaric Acid and Freeze-Dried Red Wine

Author:
Cui, Yan, Li, Qing, Zhang, Ming, Liu, Zhenzhen, Yin, Weidong, Liu, Wentao, Bi, Kaishun
Source:
Journal of agricultural and food chemistry 2010 v.58 no.23 pp. 12083–12088
ISSN:
0021-8561
Subject:
liquid chromatography, mass spectrometry, pharmacokinetics, p-coumaric acid, blood plasma, rats, animal models, freeze drying, red wines, new methods, dose response, bioavailability, equipment performance
Abstract:
A sensitive and efficient liquid chromatography−mass spectrometry (LC−MS) method was developed and validated for the determination of p-coumaric acid (CA) in rat plasma. After addition of the internal standard (IS) hydrochlorothiazide and acidification with 2 M hydrochloric acid, plasma samples were extracted by ethyl acetate and separated on a Kromasil C18 column (200 mm × 4.6 mm, 5 μm) using a mobile phase composed of methanol−0.5‰ acetic acid (60:40, v/v) within a runtime of 6.0 min. Analysis was performed in selected ion monitoring (SIM) mode with a negative electrospray ionization (ESI) interface. The target ions were m/z 163.15 for CA and m/z 295.95 for IS. The linear range was 0.01−15 μg·mL−1, and the lower limit of quantification (LLOQ) was 0.01 μg·mL−1. The intraday and interday precision (RSD %) were lower than 10% and accuracy (RE%) ranged from 97.1 to 103.2%. The validated method was successfully applied to the comparative pharmacokinetic study of CA in rat plasma after oral administration of CA and freeze-dried red wine, respectively. It was found that both AUC and T1/2 of CA in freeze-dried red wine were increased significantly (p < 0.05) compared with that in monomer. In addition, a double-peak profile could be observed from the concentration−time curve after oral administration of freeze-dried red wine.
Agid:
796425