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LC−MS Determination and Pharmacokinetics of p-Coumaric Acid in Rat Plasma after Oral Administration of p-Coumaric Acid and Freeze-Dried Red Wine

Cui, Yan, Li, Qing, Zhang, Ming, Liu, Zhenzhen, Yin, Weidong, Liu, Wentao, Bi, Kaishun
Journal of agricultural and food chemistry 2010 v.58 no.23 pp. 12083–12088
liquid chromatography, mass spectrometry, pharmacokinetics, p-coumaric acid, blood plasma, rats, animal models, freeze drying, red wines, new methods, dose response, bioavailability, equipment performance
A sensitive and efficient liquid chromatography−mass spectrometry (LC−MS) method was developed and validated for the determination of p-coumaric acid (CA) in rat plasma. After addition of the internal standard (IS) hydrochlorothiazide and acidification with 2 M hydrochloric acid, plasma samples were extracted by ethyl acetate and separated on a Kromasil C18 column (200 mm × 4.6 mm, 5 μm) using a mobile phase composed of methanol−0.5‰ acetic acid (60:40, v/v) within a runtime of 6.0 min. Analysis was performed in selected ion monitoring (SIM) mode with a negative electrospray ionization (ESI) interface. The target ions were m/z 163.15 for CA and m/z 295.95 for IS. The linear range was 0.01−15 μg·mL−1, and the lower limit of quantification (LLOQ) was 0.01 μg·mL−1. The intraday and interday precision (RSD %) were lower than 10% and accuracy (RE%) ranged from 97.1 to 103.2%. The validated method was successfully applied to the comparative pharmacokinetic study of CA in rat plasma after oral administration of CA and freeze-dried red wine, respectively. It was found that both AUC and T1/2 of CA in freeze-dried red wine were increased significantly (p < 0.05) compared with that in monomer. In addition, a double-peak profile could be observed from the concentration−time curve after oral administration of freeze-dried red wine.