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Coordinate changes in gene expression and triacylglycerol composition in the developing seeds of oilseed rape (Brassica napus) and turnip rape (Brassica rapa)

Vuorinen, Anssi L., Kalpio, Marika, Linderborg, Kaisa M., Kortesniemi, Maaria, Lehto, Kirsi, Niemi, Jarmo, Yang, Baoru, Kallio, Heikki P.
Food chemistry 2014 v.145 pp. 664-673
Brassica napus var. napus, Brassica rapa, biosynthesis, crop production, gene expression, gene expression regulation, genes, lipid content, photoperiod, seed development, seed oils, seeds, temperature, time series analysis, triacylglycerols, turnips, vegetable growing, vegetable oil
Crop production for vegetable oil in the northern latitudes utilises oilseed rape (Brassica napus subsp. oleifera) and turnip rape (B. rapa subsp. oleifera), having similar oil compositions. The oil consists mostly of triacylglycerols, which are synthesised during seed development. In this study, we characterised the oil composition and the expression levels of genes involved in triacylglycerol biosynthesis in the developing seeds in optimal, low temperature (15°C) and short day (12-h day length) conditions. Gene expression levels of several genes were altered during seed development. Low temperature and short day treatments increased the level of 9,12,15-octadecatrienoic acid (18:3n−3) in turnip rape and short day treatment decreased the total oil content in both species. This study gives a novel view on seed oil biosynthesis under different growth conditions, bringing together gene expression levels of the triacylglycerol biosynthesis pathway and oil composition over a time series in two related oilseed species.