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Cytokine expression at the anchor site in experimental Taenia solium infection in hamsters

Author:
Cruz-Rivera, Mayra, Vaughan, Gilberto, Mendlovic, Fela, Vergara-Castañeda, Arely, Romero-Valdovinos, Mirza, Leon-Cabrera, Sonia, Alonso, Monica, Avila, Guillermina, Flisser, Ana
Source:
Veterinary parasitology 2014 v.200 no.3-4 pp. 299-302
ISSN:
0304-4017
Subject:
Taenia solium, adults, animal experimentation, animal models, hamsters, humans, interferon-gamma, interleukin-10, interleukin-12, interleukin-4, messenger RNA, parasites
Abstract:
The establishment of Taenia solium adult parasite in the human intestine causes taeniosis. Importantly, the immunological mechanisms occurring at the interface between the parasite and its host are not fully known. The development of experimental animal models has facilitated the understanding of the host–parasite relationship. In this study we standardized a quantitative RT-PCR method for analyzing hamster messenger RNA for interferon-gamma (IFN-γ) and interleukins (IL): IL-4 IL-10, IL-12 and IL-13. This method was then used to evaluate the local cytokine response elicited against the adult parasite at the attachment site in the intestine of infected hamsters. The results showed an intense IFN-γ response, as well as an up-regulation of IL-4 as early as three days post-infection, permanence of IL-10 until the end of the experiment and down regulation of IL-12. These data are in agreement with a bias toward a Th-2 response as the infection progresses.
Agid:
799461