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Preparative separation of five flavones from flowers of Polygonum cuspidatum by high‐speed countercurrent chromatography

Author:
Sun, Yinshi, Gu, Shubo, Guo, Linlin, Xia, Xue, Zhang, Hong, Wang, Jianhua
Source:
Journal of separation science 2014 v.37 no.13 pp. 1703-1709
ISSN:
1615-9306
Subject:
Fallopia japonica, countercurrent chromatography, ethanol, ethyl acetate, flowers, hesperidin, luteolin, methanol, nuclear magnetic resonance spectroscopy, petroleum, phloridzin, silica gel
Abstract:
A preparative high‐speed countercurrent chromatography method was successfully used for the isolation of five minor flavones from Polygonum cuspidatum flowers. Among them, three compounds were obtained from P. cuspidatum for the first time. A twin two‐phase solvent system composed of n‐hexane/ethyl acetate/ethanol/water (1:6:3:6, v/v/v/v) and petroleum ether/ethyl acetate/methanol/water (2:4:3:3, v/v/v/v) was developed. Compounds were obtained from the fraction B and fraction C prepurified by silica gel column chromatography. Five minor compositions, 6.8 mg of hesperidin, 11.2 mg of phloridzin, 4.9 mg of luteolin, 5.3 mg of hyperin, and 3.7 mg of luteoloside were obtained from 140 mg of the fraction B and 110 mg of fraction C with a purity of 95.3, 96.4, 98.0, 96.8, and 95.3%, respectively, as determined by high‐performance liquid chromatography. The structures of these compounds were identified by ¹H and ¹³C NMR spectroscopy.
Agid:
805333