PubAg

Main content area

Mycobacterium hippocampi sp. nov., a Rapidly Growing Scotochromogenic Species Isolated from a Seahorse with Tail Rot

Author:
Balcázar, José Luis, Planas, Miquel, Pintado, José
Source:
Current microbiology 2014 v.69 no.3 pp. 329-333
ISSN:
0343-8651
Subject:
DNA, Hippocampus guttulatus, Mycobacterium, bacteria, chemotaxonomy, fatty acids, genes, pH, ribosomal RNA, sodium chloride, tail
Abstract:
A Gram-positive, aerobic, non-motile, non-sporulating, acid-fast, and rod-shaped bacterium (BFLP-6ᵀ), previously isolated from a seahorse (Hippocampus guttulatus) with tail rot, was studied using a polyphasic taxonomic approach. Growth occurred at 15–35 °C (optimum 25 °C), at pH 5.0–10.0 (optimum pH 7.0) and at NaCl concentrations between 0 and 6 % (w/v). The G+C content of DNA was 66.7 mol%. The predominant fatty acids were C₁₈:₁ ω9c, C₁₆:₀ and C₁₆:₁ ω6c. A mycolic acid pattern of alpha-mycolates and keto-mycolates was detected. Analysis of concatenated sequences (16S rRNA, rpoB, ssrA and tuf genes), and chemotaxonomic and phenotypic features indicated that strain BFLP-6ᵀ represents a novel species within the genus Mycobacterium, for which the name Mycobacterium hippocampi sp. nov. is proposed. The type strain is BFLP-6ᵀ (=DSM 45391ᵀ =LMG 25372ᵀ).
Agid:
805496