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Angiostrongylus cantonensis: Identification and characterization of microRNAs in male and female adults

Chen, Mu-Xin, Ai, Lin, Xu, Min-Jun, Zhang, Ren-Li, Chen, Shao-Hong, Zhang, Yong-Nian, Guo, Jian, Cai, Yu-Chun, Tian, Li-Guang, Zhang, Ling-Ling, Zhu, Xing-Quan, Chen, Jia-Xu
Experimental parasitology 2011 v.128 no.2 pp. 116-120
Parastrongylus cantonensis, adults, females, gene expression regulation, genes, high-throughput nucleotide sequencing, homeostasis, humans, males, meningitis, microRNA, non-coding RNA, parasitology, pathogenesis, uracil
Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and pathogenesis. They have been identified in a diverse range of organisms. The objective of this study was to determine and characterize miRNAs of female and male adults of A. cantonensis by Solexa deep sequencing. A total of 8861,260 and 10,957,957 high quality reads with 20 and 23 conserved miRNAs were obtained in females and males, respectively. No new miRNA sequence was found. Nucleotide bias analysis showed that uracil was the prominent nucleotide, particularly at positions of 1, 10, 14, 17 and 22, approximately at the beginning, middle and the end of the conserved miRNAs. To our knowledge, this is the first report of miRNA profiles in A. cantonensis, which may represent a new platform for studying regulation of genes and their networks in A. cantonensis.