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Antigen, antibody and immune complex detection in serum samples from rats experimentally infected with Strongyloides venezuelensis

Author:
Gonçalves, Ana Lúcia R., Silva, Claudio V., Ueta, Marlene T., Costa-Cruz, Julia M.
Source:
Experimental parasitology 2012 v.130 no.3 pp. 205-208
ISSN:
0014-4894
Subject:
Strongyloides venezuelensis, antibodies, antibody detection, antigen detection, antigen-antibody complex, antigens, blood serum, enzyme-linked immunosorbent assay, immunocompromised population, immunoglobulin G, parasites, parasitology, rats, screening, strongyloidiasis
Abstract:
In order to establish an antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in serum samples, normal or immunocompromised Wistar rats experimentally infected with Strongyloides venezuelensis were used. The microtitre plates were coated with IgG anti-S. venezuelensis for antigen and immune complex detection and with alkaline parasite extract for antibody detection. Analysis revealed at least 12.5μg/mL of S. venezuelensis specific antigens in serum samples. Assay for antigen detection was not a good approach for evaluating infection in normal or immunocompromised rats. In normal rats IgG specific for S. venezuelensis was preferentially detected during the first 13days post-infection (p.i.) and immune complex detection was significantly reduced in 21 p.i. day. On the other hand, in immunocompromised rats, IgG and immune complex were detected during the entire kinetic (5, 8, 13 and 21 p.i). These results suggest that immune complex screening seems to be an alternative for early strongyloidiasis diagnosis in immunocompromised individuals.
Agid:
808487