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Steady-state kinetics of tryptic hydrolysis of β-lactoglobulin after dynamic high-pressure microfluidization treatment in relation to antigenicity

Author:
Zhong, Junzhen, Luo, Shunjing, Liu, Chengmei, Liu, Wei
Source:
European food research & technology 2014 v.239 no.3 pp. 525-531
ISSN:
1438-2377
Subject:
beta-lactoglobulin, catalytic activity, digestibility, digestion, hydrolysis, proteolysis
Abstract:
Our previous research revealed that dynamic high-pressure microfluidization (DHPM) increased the antigenicity of β-lactoglobulin (β-Lg) below 80 MPa, which was related to the unfolding of protein. To test the hypothesis that the unfolding of protein may change proteolytic susceptibility of β-Lg and modulate its antigenicity during the digestion, we developed that the steady-state kinetics of tryptic hydrolysis of β-Lg subjected to DHPM (0.1–80 MPa) have been investigated in relation to the antigenicity in this study. According to the steady-state kinetics analysis, the improved digestion of β-Lg was accompanied with the obvious decrease of antigenicity during the hydrolysis with pressure increasing, reflected by the increase of k c , the decrease of Kₘ, the increase of overall catalytic efficiency (kc/Kₘ), and the increase of the binding volume. It was indicated that although DHPM can increase the antigenicity of β-Lg, the enhanced digestibility of β-Lg at elevated pressure contributed to a decrease of antigenicity during the hydrolysis.
Agid:
810403