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Characterization and evaluation of microsatellite loci in European hazelnut (Corylus avellana L.) and their transferability to other Corylus species

Author:
Boccacci, P., Akkak, A., Bassil, N.V., Mehlenbacher, S.A., Botta, R.
Source:
Molecular ecology notes 2005 v.5 no.4 pp. 934
ISSN:
1471-8278
Subject:
Corylus avellana, Corylus, hazelnuts, microsatellite repeats, genetic markers, loci, nucleotide sequences, polymerase chain reaction, DNA primers, heterozygosity, alleles
Abstract:
In this work, 18 microsatellite loci were developed in the European hazelnut (Corylus avellana L.) using three enriched genomic libraries. They were evaluated on a set of 20 accessions of this species on the basis of number of alleles (mean: 7.1), expected heterozygosity (mean: 0.67), power of discrimination (mean: 0.77) and polymorphism information content (mean: 0.64). Cross-species transferability was evaluated using seven other Corylus species. All primer pairs amplified in all species, except for CaT-C505 in Corylus ferox and CaT-A114 in Corylus californica.
Agid:
8110
Handle:
10113/8110