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Expression of OsAMT1 (1.1-1.3) in rice varieties differing in nitrogen accumulation

Zhao, S. P., Ye, X. Z., Shi, W. M.
Russian journal of plant physiology 2014 v.61 no.5 pp. 707-713
Oryza sativa, ammonium nitrate, cultivars, genes, genotype, nitrogen, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, rice, roots, seedlings
OsAMT is a high-affinity ammonium transporter responsible for NH ₄ ⁺ uptake by rice plants. To investigate the expression patterns of OsAMT in different genotypes in relation to nitrogen accumulation, we measured the expression of OsAMT1.1, OsAMT1.2, and OsAMT1.3 using Real-Time PCR (RT-PCR) in GD (higher N accumulation) and NG (lower N accumulation) seedlings of the Oryza sativa L. cultivar treated with 0.1 mM NH₄NO₃ and 2 mM NH₄NO₃. We found that the expression level of OsAMT1.1 was significantly higher than those of OsAMT1.2 and OsAMT1.3 in the roots treated with 0.1 mM NH₄NO₃, suggesting that OsAMT1.1 contributed the most to N accumulation among the three genes. In GD root, OsAMT1.1 had significantly higher expression levels when it was up-regulated by 0.1 mM NH₄NO₃ than when down-regulated by 2 mM NH₄NO₃. OsAMT1.1 was mainly found in GD roots treated with 0.1 mM NH₄NO₃. We conclude that the OsAMT1.1 in GD roots, which was significantly up-regulated by low N and down-regulated by high N, was the dominating factor in determining the higher N acquisition in GD than in NG at 0.1 mM NH₄NO₃.