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Antifungal efficiency assessment of the TiO₂ coating on façade paints

Vučetić, Snežana B., Rudić, Ognjen Lj., Markov, Siniša L., Bera, Oskar J., Vidaković, Ana M., Skapin, Andrijana S. Sever, Ranogajec, Jonjaua G.
Environmental science and pollution research international 2014 v.21 no.19 pp. 11228-11237
Aspergillus niger, agar, antifungal properties, coatings, fungi, hydroxides, hyphae, microbial growth, monitoring, mycelium, nanocomposites, paints, photolysis, spores, titanium dioxide, ultraviolet radiation
The work studies the photocatalytic activity and the antifungal efficiency of the TiO₂/Zn-Al coatings placed on the target commercial façade paints. The photocatalytic active nanocomposite based on TiO₂ and Zn-Al-layered double hydroxides (ZnAl-LDHs) was synthesized by a wet impregnation technique with 3 % w/w TiO₂. The freshly prepared suspension was applied by spray technique on the surfaces of the white façade paints. The goal of the work was to develop a method that quickly quantifies the antifungal activity of the commercial façade paints with and without biocidal components covered with a photocatalytic coating. The essence of the proposed method is the monitoring of the fungal growth (artificial ageing conditions) and the quantification of its development (UV-A 0.13 mWcm⁻²) on the façade paint surfaces. A special fungus nutrient (potato dextrose agar (PDA)) was inoculated with the spores of the Aspergillus niger ATCC 6275, and the test samples (façade paints with and without photocatalytic coating) were placed on the inoculated nutrient in the petri dishes. The images of the fungal growth on the samples of the facade paints, during a period of 5 days, were imported into Matlab R2012a where they were converted to binary images (BW), based on the adequate threshold. The percentage of the surface coverage was calculated by applying the specifically written program code which determines the ratio of the black and white pixels. The black pixels correspond to the surface covered with hyphae and mycelia of the fungus.