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Complete NMR assignments of undegraded asterosaponins from Asterias amurensis

Author:
Hwang, In Hyun, Kulkarni, Roshan, Yang, Min Hye, Choo, Soo Jin, Zhou, Wei, Lee, Sang Myung, Jang, Tae Su, Jeong, Gil-Saeng, Chang, Hyeun Wook, Na, MinKyun
Source:
Archives of pharmacal research 2014 v.37 no.10 pp. 1252-1263
ISSN:
0253-6269
Subject:
Asterias, bioactive properties, chemical degradation, magnetic fields, nuclear magnetic resonance spectroscopy, secondary metabolites, steroid saponins
Abstract:
Four asterosaponins, thornasteroside A (1), versicoside A (2), anasteroside B (3), and asteronylpentaglycoside sulfate (4), were isolated from the predatory starfish Asterias amurensis Lütken. Unlike previous studies focusing on structure elucidation by degradation of the complex saponin molecules, complete nuclear magnetic resonance (NMR) assignment for the intact molecules was accomplished using 600 MHz high magnetic field NMR. The complete set of NMR assignments can help in the structure elucidation of asterosaponins isolated in low yields without resorting to chemical degradation. Furthermore, this approach can be extended to other complex steroidal saponins, which may accelerate the discovery of bioactive secondary metabolites from this invasive starfish species.
Agid:
818880