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Operational Stability and Kinetic Study of a Membrane Reactor with Pectinases from Aspergillus niger
- Rodríguez‐Nogales, J.M., Ortega, N., Perez‐Mateos, M., Busto, M.D.
- Journal of food science 2005 v.70 no.2 pp. E104
- Aspergillus niger, catalytic activity, equations, half life, hydrolysis, models, pH, pectins
- The kinetic and operational behavior of a free enzyme membrane reactor (FEMR) were analyzed. High conversion (83%) of the pectin could be achieved with a substrate‐to‐enzyme ratio of 23.3 at 46°C and pH 4.8, with a transmembrane pressure of 34.5 kPa and recycling flow rate of 36 L/h. The FEMR showed a 1st‐order deactiva‐tion profile with a calculated half life (t₁/₂) of 21.7 d in the operation conditions. Akinetic study of the hydrolysis of pectin by free endopectinases from Aspergillus niger was done by the simultaneous use of a stirred‐tank reactor and a FEMR. Kinetic data were fit to a model based on the Michaelis‐Menten rate expression and the design equation for a continuous membrane reactor. The kinetic characteristics observed in the hydrolysis of pectin included a product competitive inhibition. The Kₘ and Kᵢ values for the membrane reactor were 2 to 3 times greater those obtained in a stirred‐tank reactor. On the basis of the Vₘₐₓ values, the configuration of the FEMR was found to have excellent catalytic efficiency when compared with the stirred‐tank reactor.