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Operational Stability and Kinetic Study of a Membrane Reactor with Pectinases from Aspergillus niger

Rodríguez‐Nogales, J.M., Ortega, N., Perez‐Mateos, M., Busto, M.D.
Journal of food science 2005 v.70 no.2 pp. E104
Aspergillus niger, catalytic activity, equations, half life, hydrolysis, models, pH, pectins
The kinetic and operational behavior of a free enzyme membrane reactor (FEMR) were analyzed. High conversion (83%) of the pectin could be achieved with a substrate‐to‐enzyme ratio of 23.3 at 46°C and pH 4.8, with a transmembrane pressure of 34.5 kPa and recycling flow rate of 36 L/h. The FEMR showed a 1st‐order deactiva‐tion profile with a calculated half life (t₁/₂) of 21.7 d in the operation conditions. Akinetic study of the hydrolysis of pectin by free endopectinases from Aspergillus niger was done by the simultaneous use of a stirred‐tank reactor and a FEMR. Kinetic data were fit to a model based on the Michaelis‐Menten rate expression and the design equation for a continuous membrane reactor. The kinetic characteristics observed in the hydrolysis of pectin included a product competitive inhibition. The Kₘ and Kᵢ values for the membrane reactor were 2 to 3 times greater those obtained in a stirred‐tank reactor. On the basis of the Vₘₐₓ values, the configuration of the FEMR was found to have excellent catalytic efficiency when compared with the stirred‐tank reactor.