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A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens

Author:
Pongsomboon, Siriporn, Tang, Sureerat, Boonda, Suleeporn, Aoki, Takashi, Hirono, Ikuo, Tassanakajon, Anchalee
Source:
Fish & shellfish immunology 2011 v.30 no.1 pp. 439-446
ISSN:
1050-4648
Subject:
Gill-associated virus, Penaeus monodon, Vibrio harveyi, White spot syndrome virus, complementary DNA, disease course, expressed sequence tags, gene expression, gene expression regulation, genes, hemocytes, immune response, microarray technology, pathogens, shrimp, transcription (genetics), vibriosis, viruses, white spot syndrome
Abstract:
A cDNA microarray comprised of 9990 different ESTs obtained from the Penaeus monodon EST project (http://pmonodon.biotec.or.th) was employed to identify viral (white spot and yellow head viruses) and bacterial (Vibrio harveyi) responsive genes in the hemocytes of P. monodon at 6, 24 and 48 h post-injection (hpi). The number of differentially expressed genes found was highest in shrimps infected with white spot virus (1954 genes) followed by yellow head virus (1136 genes) and V. harveyi (420 genes). Changes in shrimp gene expression were highest at the late infection stage for both viruses, whilst that for V. harveyi induced gene expression was mainly found at the early infection stage, but the repression of genes was mainly found in the mid stage of infection. Shrimp genes specifically upregulated by each particular pathogen are identified and are summarized.
Agid:
828636