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Molecular cloning and characterization of European seabass (Dicentrarchus labrax) and Gilthead seabream (Sparus aurata) complement component C3

Author:
Mauri, I., Roher, N., MacKenzie, S., Romero, A., Manchado, M., Balasch, J.C., Béjar, J., Álvarez, M.C., Tort, L.
Source:
Fish & shellfish immunology 2011 v.30 no.6 pp. 1310-1322
ISSN:
1050-4648
Subject:
Dicentrarchus labrax, Sparus aurata, complement, complementary DNA, cysteine, genes, molecular cloning, nucleotide sequences, pathogens, polymerase chain reaction, proteins, tissue distribution
Abstract:
We present the complete C3 cDNA sequence of Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) and its molecular characterization with a descriptive analysis of their structural elements. We obtained one sequence for Gilthead seabream (gsbC3) which encodes a predicted protein of 1656 amino acids, and two sequences for European seabass (esbC3_1 and esbC3_2) which encode two predicted proteins of 1654 and 1587 amino acids respectively. All sequences present the characteristic structural features of C3 but interestingly esbC3_2 lacks the anaphylotoxin domain and the cysteine residue responsible for thiolester bond formation. Moreover, we have detected and quantified (by real-time PCR-based absolute quantification) specific isoform expression in European seabass depending on pathogen and density conditions in vivo. In addition, we have analyzed the tissue distribution pattern of European seabass and Gilthead seabream C3 genes under crowding stress and under pathological challenges in vivo, and we have observed that crowding and infection status provoke changes in expression levels, tissue expression pattern and C3 isoform expression balance.
Agid:
828746