Jump to Main Content
Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property
- Sun, Jin-sheng, Zhao, Lu, Sun, Li
- Fish & shellfish immunology 2011 v.30 no.6 pp. 1362-1367
- Cynoglossus semilaevis, amino acid sequences, cell culture, chemoattractants, cysteine, dose response, fish, gene expression regulation, inflammation, interleukin-1beta, interleukin-8, kidneys, lymphocytes, mammals, reverse transcriptase polymerase chain reaction, subunit vaccines, tissues, vaccination
- Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1β and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response.