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Facile synthesis of metal-chelating magnetic nanoparticles by exploiting organophosphorus coupling

Author:
Yang, Kun, Su, Wei Wen
Source:
Analytical biochemistry 2011 v.408 no.1 pp. 175-177
ISSN:
0003-2697
Subject:
aqueous solutions, bacteria, binding capacity, chelating agents, chloramphenicol acetyltransferase, green fluorescent protein, ions, iron oxides, mixing, nanoparticles, nickel, organophosphorus compounds, protein binding
Abstract:
A new method is described for facile synthesis of metal-chelating magnetic nanoparticles by simply mixing iron oxide nanoparticles with a bifunctional organophosphorus compound, N-(phosphonomethyl)iminodiacetic acid (PM–IDA), in aqueous solution. On charging with nickel ions, the PM–IDA functionalized iron oxide nanoparticles exhibited high His-tag protein binding capacity (0.21 and 0.58mg/mg for His-tagged green fluorescent protein and chloramphenicol acetyltransferase, respectively) and were successfully used to purify these proteins from bacterial cell extracts to high purity in a single step. Although other synthetic schemes for metal-chelating magnetic nanoparticles have been reported, the method described here is markedly simpler and involves only low-cost reagents.
Agid:
829872