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Development and application of a novel histological multichrome technique for clam histopathology
- Costa, Pedro M., Costa, Maria H.
- Journal of invertebrate pathology 2012 v.110 no.3 pp. 411-414
- Perkinsus, Ruditapes decussatus, clams, histology, histopathology, polysaccharides, staining, tissues
- A novel and expedient histological tetrachrome technique was developed and applied to whole-body sections of the clam Ruditapes decussatus (L. 1758). The technique involves fixation in Carnoy’s fluid followed by immediate embedding in paraffin with staining with a combination of Alcian Blue, Periodic Acid–Schiff’s, Haematoxylin and Picric Acid. Fixation and staining was perfect for all tissues and resolved good identification of Perkinsus sp. infection and high structural detail. Among the surveyed fixatives, Bouin–Hollande’s fluid also provided good results, however, fixation is potentially longer, polysaccharide staining was less intense and fibres appeared to be better preserved by Carnoy’s.