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Characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate that does not liquefy the integument of infected larvae
- Vieira, Corina M., Tuelher, Edmar S., Valicente, Fernando H., Wolff, José Luiz Caldas
- Journal of invertebrate pathology 2012 v.111 no.2 pp. 189-192
- Spodoptera frugiperda multiple nucleopolyhedrovirus, biopesticides, chitinase, frameshift mutation, genes, instars, integument, larvae, lethal concentration 50, liquefaction, virulence
- Here we report the characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate, named SfMNPV-6nd, that does not cause the liquefaction of the host integument. The sequencing of the chitinase A (v-chiA) gene from SfMNPV-6nd revealed that it had a frameshift mutation that greatly reduced size of the putative enzyme. In order to evaluate the suitability of SfMPNV-6nd as a biopesticide, this isolate was compared with the highly virulent SfMNPV-19. Our results showed that the LC₅₀ of the two isolates were not significantly different, but that SfMNPV-6nd took a longer period of time to kill second instar S. frugiperda than SfMNPV-19.