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Comparing two methods for quantifying soil-borne Entomophaga maimaiga resting spores

Author:
Hajek, Ann E., Plymale, Ruth C., Reilly, James R.
Source:
Journal of invertebrate pathology 2012 v.111 no.2 pp. 193-195
ISSN:
0022-2011
Subject:
Entomophaga maimaiga, Lymantria dispar, equations, fungal spores, pathogens, prediction, soil, soil fungi
Abstract:
To improve usability of methods for quantifying environmentally persistent entomophthoralean resting spores in soil, we modified and tested two methods using resting spores (azygospores) of the gypsy moth pathogen Entomophaga maimaiga. Both methods were effective for recovering resting spores at concentrations >100 resting spores/g dry soil. While a modification of a method originally described by Weseloh and Andreadis (2002) recovered more resting spores than a modified method based on Percoll density gradients, the ability to estimate true densities from counts was similar for both methods. Regression equations are provided for predicting true resting spore densities from counts, with R² values for both methods ⩾0.90.
Agid:
832342