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Growth and differentiation characteristics of equine mesenchymal stromal cells derived from different sources

Author:
Burk, Janina, Ribitsch, Iris, Gittel, Claudia, Juelke, Henriette, Kasper, Cornelia, Staszyk, Carsten, Brehm, Walter
Source:
The veterinary journal 2013 v.195 no.1 pp. 98-106
ISSN:
1090-0233
Subject:
adipocytes, adipose tissue, blood, bone formation, bone marrow, chondrocytes, collagen, horses, osteoblasts, stromal cells, umbilical cord
Abstract:
Multipotent mesenchymal stromal cells (MSCs) are a promising therapeutic tool for the treatment of equine tendon and other musculoskeletal injuries. While bone marrow is considered the ‘gold standard’ source of these cells, various other tissues contain MSCs with potentially useful features. The aim of this study was to compare clinically relevant characteristics of MSCs derived from bone marrow, umbilical cord blood and tissue and from adipose tissue and tendon. Cell yield, proliferation, migration, tendon marker expression and differentiation into adipocytes, chondrocytes and osteoblasts was assessed, quantified and compared.MSC numbers obtained from adipose, tendon or umbilical cord tissues were 222-fold higher than those obtained from bone marrow or cord blood. Cells derived from tendon and adipose tissues exhibited most rapid proliferation. Osteogenic differentiation was most prominent in MSCs derived from bone marrow, and was weak in MSCs derived from umbilical cord blood and tissue. In contrast, the highest levels of chondrogenic differentiation were observed in MSCs derived from these sources. Collagen 1A2 expression was highest in adipose- and tendon-derived MSCs, while scleraxis expression was highest in cord blood- and in tendon-derived MSCs. The findings indicate that MSCs from different sources display significantly diverse properties that may impact on their therapeutic application.
Agid:
836189