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Genetic relationship among Diabrotica species (Coleoptera: Chrysomelidae) based on rDNA and mtDNA sequences

Szalanski, A.L., Roehrdanz, R.L., Taylor, D.B.
Florida entomologist 2000 v.83 no.3 pp. 262
genes, cytochrome-c oxidase, Diabrotica virgifera virgifera, Diabrotica barberi, Diabrotica undecimpunctata howardi, Diabrotica balteata, ribosomal DNA, mitochondrial DNA, nucleotide sequences, genetic distance, phylogeny, Diabrotica virgifera zeae, intergenic DNA
Corn rootworms of the genus Diabrotica (Coleoptera: Chrysomelidae) are the most serious pest of maize in the midwestern United States. Despite their economic importance, phylogenetic relationships within the genus remain unclear. Phylogenetic analysis of five Diabrotica species and subspecies (D. virgifera virgifera, D. v. zeae, D. barberi, D. balteata and D. undecimpunctata howardi) was undertaken using DNA sequences of the nuclear rDNA first internal transcribed spacer region (ITS1) and a portion of the mtDNA cytochrome oxidase I and II genes (COI/COII). Parsimony and maximum likelihood analysis indicated that southern corn rootworm (D. u. howardi) is sister to banded cucumber beetle (D. balteata), whereas, northern corn rootworm (D. barberi) forms a distinct clade with western and Mexican corn rootworms (D. v. virgifera and D. v. zeae, respectively). ITS1 and COI/COII were found to be useful markers for determining phylogenetic relationships among diabroticites.