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Chromatographic analysis, anti-proliferative and radical scavenging activity of Pinus wallichina essential oil growing in high altitude areas of Kashmir, India

Author:
Yousuf Dar, Mohd, Shah, Wajaht A., Mubashir, Sofi, Rather, Manzoor A.
Source:
Phytomedicine 2012 v.19 no.13 pp. 1228-1233
ISSN:
0944-7113
Subject:
Pinus wallichiana, adenocarcinoma, alpha-pinene, altitude, animal ovaries, anticarcinogenic activity, beta-pinene, disease control, drugs, essential oils, free radical scavengers, gas chromatography-mass spectrometry, humans, hydrodistillation, iodine, liver, lung neoplasms, monocytes, nuclear magnetic resonance spectroscopy, oils, ovarian neoplasms, reactive oxygen species, silica gel, India
Abstract:
PURPOSE: To evaluate the in vitro anti-proliferative and radical scavenging properties of the essential oil and its fractions and to determine the chemo-type of P. wallichiana essential oil. METHOD: Pinus wallichiana oil was extracted by hydro-distillation and fractionated by silica gel column chromatography method. The oil and its fractions were analyzed by Gas chromatography, Gas chromatography–mass spectrometry and ¹³C NMR. Different concentrations of oil 12.5, 25, 50 and 100μg/ml and single concentration 50μg/ml of its fractions B₁, B₂, A₂, G₂, Uk₁₃ and I₂ were evaluated for its anti-proliferative activity by in vitro {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide} assay against human monocyte, lung carcinoma, liver adenocarcinoma, prostate and ovarian carcinoma, while as the radical scavenging activity was evaluated by different in vitro DPPH assays. RESULTS: The analyses indicated the presence of 17 constituents with β-pinene (46.8%) and α-pinene (25.2%) as major constituents. The oil and its fractions showed significant anti-proliferative activity. The radical scavenging activity also showed good results. CONCLUSION: The oil could be used as a drug to control the diseases like cancer, cirrhosis and arteriosclerosis, caused by reactive oxygen species.
Agid:
839105