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Characterization of a novel temperate phage originating from a cereulide-producing Bacillus cereus strain

Smeesters, Pierre R., Drèze, Pierre-Alexandre, Bousbata, Sabrina, Parikka, Kaarle J., Timmery, Sophie, Hu, Xiaomin, Perez-Morga, David, Deghorain, Marie, Toussaint, Ariane, Mahillon, Jacques, Van Melderen, Laurence
Research in microbiology 2011 v.162 no.4 pp. 446-459
Bacillus cereus, Bacillus thuringiensis, Siphoviridae, bacteriophages, databases, interspersed repetitive sequences, mass spectrometry, morphogenesis, open reading frames, plasmids, sequence analysis, structural proteins
A novel temperate bacteriophage was isolated from a Bacillus cereus cereulide-producing strain and named vB_BceS-IEBH. vB_BceS-IEBH belongs to the Siphoviridae family. The complete genome sequence (53kb) was determined and annotated. Eighty-seven ORFs were detected and for 28, a putative function was assigned using the ACLAME database. vB_BceS-IEBH replicates as a plasmid in the prophage state. Accordingly, a 9-kb plasmid-like region composed of 13 ORFs was identified. A fragment of around 2000bp comprising an ORF encoding a putative plasmid replication protein was shown to be self-replicating in Bacillus thuringiensis. Mass spectrometry analysis of the purified vB_BceS-IEBH particle identified 8 structural proteins and enabled assignment of a supplementary ORF as being part of the morphogenesis module. Genome analysis further illustrates the diversity of mobile genetic elements and their plasticity within the B. cereus group.