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Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)

Decat, Ellen, Van Mechelen, Els, Saerens, Bart, Vermeulen, Stefan J.T., Boekhout, Teun, De Blaiser, Steven, Vaneechoutte, Mario, Deschaght, Pieter
Research in microbiology 2013 v.164 no.2 pp. 110-117
Candida albicans, Candida glabrata, Candida kefyr, Candida krusei, Candida parapsilosis, Candida saitoana, Candida tropicalis, Clavispora lusitaniae, Meyerozyma guilliermondii, Saccharomyces cerevisiae, Yarrowia lipolytica, cost effectiveness, melting, therapeutics, yeasts
Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishing between 16 Candida spp., i.e. Candida albicans, Candida bracarensis, Candida dubliniensis, Candida famata, Candida glabrata, Candida guilliermondii, Candida inconspicua, Candida kefyr, Candida krusei, Candida lipolytica, Candida lusitaniae, Candida nivariensis, Candida norvegensis, Candida parapsilosis, Candida tropicalis and Candida sojae, and Saccharomyces cerevisiae and one species pair, i.e. Candida metapsilosis/Candida orthopsilosis. Starting from a cultured isolate, ITS2-MCA led to differentiation of these species within 6 h. According to our findings, ITS2-MCA offers a simple, rapid and cost-effective method for identification of cultured isolates of the clinically most relevant and prevalent Candida species. Further studies will be necessary to evaluate how it performs on mixed samples and clinical samples.