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An improved protocol for electroporation in members of the genus Gordonia

Author:
Singh, Pooja, Srivastava, Preeti
Source:
Journal of microbiological methods 2013 v.95 no.2 pp. 114-116
ISSN:
0167-7012
Subject:
Gordonia amarae, Gordonia rubripertinctus, Gram-positive bacteria, electroporation
Abstract:
Gordonia are high GC gram-positive bacteria that have not yet been exploited well for biotechnological purposes because of the limited genetic tools. Described here is an improved protocol for electroporation, which is useful for several Gordonia species. The maximum transformation efficiency obtained was 2.8×10⁴/μg (Gordonia rubropertinctus, Gordonia sp), and 1.7×10³/μg (Gordonia amarae).
Agid:
848265