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Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria
- Britos, Claudia N., Cappa, Valeria A., Rivero, Cintia W., Sambeth, Jorge E., Lozano, Mario E., Trelles, Jorge A.
- Journal of molecular catalysis 2012 v.79 pp. 49-53
- Lactobacillus animalis, adsorption, bioprocessing, biotransformation, calcium alginate, catalytic activity, floxuridine, lactic acid bacteria, microbial load
- An efficient and green bioprocess is herein reported to obtain halogenated nucleosides by transglycosylation using immobilized lactic acid bacteria (LAB). Lactobacillus animalis ATCC 35046 showed a yield of 95% at 0.5h to synthesize 5-fluorouracil-2′-deoxyriboside (floxuridine). Calcium alginate was the best matrix for whole-cell immobilization by entrapment. Its productivity was 87mg/Lh in a continuous bioprocess. When adsorption techniques were evaluated, DEAE-Sepharose was the support which showed higher microbial load, its productivity being 53mg/Lh. Additionally, this microorganism was able to produce 5-bromouracil-2′-deoxyriboside, 6-chloropurine-2′-deoxyriboside and 6-bromopurine-2′-deoxyriboside.