U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Enzymatic transformation of tyrosol by Trametes trogii laccases: Identification of the product and study of its biological activities

Hanen Chakroun, Mohamed Bouaziz, Thabèt Yangui, Imen Blibech, Abdelhafidh Dhouib, Sami Sayadi
Journal of Molecular Catalysis. B, Enzymatic 2013 v.87 pp. 11-17
Enterococcus hirae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Trametes trogii, Tuta absoluta, antifungal properties, aqueous solutions, carbon, catalytic activity, enzymatic reactions, insecticidal properties, insects, laccase, nuclear magnetic resonance spectroscopy, pests, phytotoxicity, stable isotopes, tomatoes, viability
Oxidative transformation of tyrosol catalysed by Trametes trogii laccases in aqueous solution was investigated. LC–MS analysis shows that tyrosol was converted to its dimer. The enzymatic reaction was also investigated by 1H and 13C nuclear magnetic resonance, and the product formed was identified as a dimeric tetracyclic ketone. The bactericidal and fungicidal properties of tyrosol dimer were investigated using the NCCLS broth dilution and EN 1276 standard methods. High bactericidal and fungicidal effect of concentrations ranged between 1–0.5gL−1 and 8–4gL−1 were obtained. Dimer concentrations of 33gL−1 and 66gL−1 allowed reductions in viability higher than 5log units per mL for Pseudomonas aeruginosa ATCC 15442, Escherichia coli ATCC 10536 and Enterococcus hirae ATCC 10541, Staphylococcus aureus ATCC 9144 respectively, within a contact time of 5min under dirty conditions. The effect of this product on Tuta absoluta, a harmful pest of tomato in the world, was also evaluated. The results showed high insecticidal activity against this insect at a concentration of 16.5gL−1. Germinability experiments on Lycopersicum esculetum were conducted in order to evaluate the potential of a laccase treatment in removing tyrosol phytotoxicity. The results showed that tyrosol dimer was nonphytotoxic. This study presents the first comprehensive results of biological characterisation of the product obtained by the action of laccase on tyrosol transformation with T. trogii laccases.