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The use of soybean peroxidase in the decolourization of Remazol Brilliant Blue R and toxicological evaluation of its degradation products

Silva, Maria Cristina, Torres, Juliana Arriel, Vasconcelos de Sá, Lívian Ribeiro, Chagas, Pricila Maria Batista, Ferreira-Leitão, Viridiana Santana, Corrêa, Angelita Duarte
Journal of Molecular Catalysis. B, Enzymatic 2013 v.89 pp. 122-129
Artemia salina, Lactuca sativa, catalytic activity, decolorization, enzymatic treatment, fabrics, hydrogen peroxide, inhibitory concentration 50, lethal concentration 50, lettuce, peroxidase, seeds, toxicity
This study evaluated the potential use of soybean peroxidase in the decolourization of reactive textile dye Remazol Brilliant Blue R (RBBR) and its synthetic effluent. The following parameters were studied: reaction time, dye concentration (10–60mgL−1), enzyme load (4.96–140UmL−1) and H2O2 concentration (20–1100μmolL−1). The maximum removal of RBBR (86%) was obtained after 13min of reaction, using H2O2 100μmolL−1, enzyme 70.4UmL−1 and RBBR 40mgL−1. The toxicity of the products formed after enzymatic treatment was assessed by using Artemia salina and lettuce seeds (Lactuca sativa). Although soybean peroxidase was very efficient in colour removal, the products obtained after enzymatic decolourization presented higher toxicity. The inhibition concentration (IC50) obtained for lettuce seeds was 27.9%, and the lethal concentration (LC50) for A. salina was 59.3%. The aforementioned results emphasize the importance of toxicological evaluation after enzymatic treatment. The potential application of peroxidases for colour removal and the increase in the products’ toxicity reinforce the need of combined treatments.