Jump to Main Content
Studies toward stereoselective bionanocatalysis on gold nanoparticles
- Jędrzejewska, Hanna, Ostaszewski, Ryszard
- Journal of Molecular Catalysis. B, Enzymatic 2013 v.90 pp. 12-16
- nanogold, enantiomers, Burkholderia cepacia, immobilized enzymes, biocatalysis, nanoparticles, adsorption
- As yet, different enzymes were immobilized on gold nanoparticles both through adsorption and covalent binding. However, there is only one evaluation if such immobilization influenced enzyme enantioselectivity, which is an essential parameter in biocatalysis. Therefore systematic studies with enzymes immobilized on gold nanoparticles through covalent binding and embedded through adsorption were performed. Adsorption was not efficient method and it significantly lowered enantioselectivity of enzymes. In turn, covalent binding was in most cases very good method of immobilization, especially for Pseudomonas cepacia lipase, where conversion and enantioselectivity were even slightly better than for native enzyme. It was also evaluated that in case of adsorption size of nanoparticles did not influence enantioselectivity, but in case of covalent binding small nanoparticles gave much better results than big ones.