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Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine

Zhang, Jie, Zhou, Xiaohua, Wang, Dan, Wang, Yingli, Zhou, Xing, Wang, Honghui, Li, Qiang, Tan, Shiyu
Journal of Molecular Catalysis. B, Enzymatic 2013 v.97 pp. 80-86
catalase, catalytic activity, chitosan, crosslinking, glucose, glucose oxidase, heat stability, hydrogen peroxide, immobilized enzymes, lysine, pH, temperature
Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on a novel crosslinked chitosan resin modified by l-lysine. The immobilized system exhibited the best performance when the immobilization process was conducted at 4°C for 3h with GOD/CAT activity ratio of 1:18 and pH 7.0. Under the optimum pH 4.0 and temperature 40°C, the enzyme system could achieve to the maximum conversion of glucose, which was 68.9%. Acidoresistance and thermotolerance of the enzymes were both strengthened. The Km of the co-immobilized enzyme system obtained in this study was 31.02mM. The GOD enzyme immobilized in this system could maintain 94% of initial activity after the 12th operational cycle. 95.8% of initial activity was left after 50 weeks’ storage at 4°C. GOD achieved better performance in this new immobilized enzyme system due to its effective elimination of H2O2, which showed potential applications for various commercial uses.