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Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine
- Zhang, Jie, Zhou, Xiaohua, Wang, Dan, Wang, Yingli, Zhou, Xing, Wang, Honghui, Li, Qiang, Tan, Shiyu
- Journal of Molecular Catalysis. B, Enzymatic 2013 v.97 pp. 80-86
- catalase, catalytic activity, chitosan, crosslinking, glucose, glucose oxidase, heat stability, hydrogen peroxide, immobilized enzymes, lysine, pH, temperature
- Glucose oxidase (GOD) and catalase (CAT) were co-immobilized on a novel crosslinked chitosan resin modified by l-lysine. The immobilized system exhibited the best performance when the immobilization process was conducted at 4°C for 3h with GOD/CAT activity ratio of 1:18 and pH 7.0. Under the optimum pH 4.0 and temperature 40°C, the enzyme system could achieve to the maximum conversion of glucose, which was 68.9%. Acidoresistance and thermotolerance of the enzymes were both strengthened. The Km of the co-immobilized enzyme system obtained in this study was 31.02mM. The GOD enzyme immobilized in this system could maintain 94% of initial activity after the 12th operational cycle. 95.8% of initial activity was left after 50 weeks’ storage at 4°C. GOD achieved better performance in this new immobilized enzyme system due to its effective elimination of H2O2, which showed potential applications for various commercial uses.