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A novel lipase-based stationary phase in liquid chromatography
- He, Zeying, Lv, Chunguang, Fan, Xinxin, Zhou, Zhiqiang
- Analytica chimica acta 2011 v.689 no.1 pp. 143-148
- Pseudozyma antarctica, alcohols, bioreactors, diniconazole, enantiomers, epoxides, esters, high performance liquid chromatography, hydrolysis, immobilized enzymes, silica gel
- In this paper, a new chiral stationary phase (CSP) based on Candida antarctica lipase B (CALB) bounding to the surface of macroporous silica gel was developed and its stereoselectivity in enantioseparation and asymmetrical hydrolysis was evaluated. Three CALB-based HPLC columns with different amounts of enzyme immobilized were prepared by employing the immobilization method, namely “in batch”. In this technique two chromatographic supports epoxy silica and aminopropyl silica were considered. This novel CSP was proven capable of hydrolyzing chiral esters asymmetrically as bioreactor and separating several aromatic alcohols and diniconazole enantiomers.