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Development and validation of an open screening method for diuretics, stimulants and selected compounds in human urine by UHPLC–HRMS for doping control

Author:
Jiménez Girón, A., Deventer, K., Roels, K., Van Eenoo, P.
Source:
Analytica chimica acta 2012 v.721 pp. 137-146
ISSN:
0003-2670
Subject:
detection limit, diuretics, humans, mass spectrometry, screening, urine
Abstract:
A new doping control screening method for the analysis of diuretics and stimulants using ultra high pressure liquid chromatography–high resolution Orbitrap mass spectrometry has been developed. The screening was performed in full scan MS with scan-to-scan polarity switching which allowed to detect more than 120 target analytes. Sample preparation was limited to 10-fold dilution of the urine into the internal standard solution followed by injection. Total run time per sample was 10min. Validation of the method yielded detection limits for diuretics between 25 and 250ngmL⁻¹ and for stimulants between 5 and 500ngmL⁻¹. The screening method has been implemented in routine doping control.
Agid:
857616