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Determination of amino acids in selenium-enriched yeast by gas chromatography–mass spectrometry after microwave assisted hydrolysis

Author:
Zhang, Xu, Yang, Lu, Mester, Zoltan
Source:
Analytica chimica acta 2012 v.744 pp. 54-59
ISSN:
0003-2670
Subject:
amino acids, chloroform, detection limit, foods, gas chromatography-mass spectrometry, heat, hydrolysis, microwave treatment, monitoring, temperature, yeasts
Abstract:
A simple, rapid microwave digestion procedure for protein hydrolysis preceding the determination of amino acids in yeast using gas chromatography–mass spectrometry (GC–MS) is described. Protein hydrolysis was performed in a focused microwave using 4M methanesulfonic acid (MAS). Amino acids were derivatized with methyl chlorofomate (MCF) and extracted into chloroform prior to GC–MS analysis. The microwave parameters, including power, temperature and heating time, were optimized. It was found that temperature and heating time were the most influential factors. A total of 17 amino acids were determined in selenium-enriched yeast with use of standard addition calibration. Limits of detection and quantitation (LODs/LOQs) of the amino acids measured were in the sub-nmol range, suitable for monitoring of amino acids in yeast and other food products.
Agid:
857934