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Corosolic acid induces apoptotic cell death in human lung adenocarcinoma A549 cells in vitro

Nho, Kyoung Jin, Chun, Jin Mi, Kim, Ho Kyoung
Food and chemical toxicology 2013 v.56 pp. 8-17
NAD ADP-ribosyltransferase, acetylcysteine, adenocarcinoma, apoptosis, caspases, cell cycle, cell viability, humans, medicinal plants, proteins, reactive oxygen species, toxicology
Corosolic acid (CRA), a triterpenoid from medicinal herbs, has been shown to induce apoptosis in several cell lines, with the exception of A549 cells. In this report, we investigated the apoptotic effect and mechanism of CRA in A549 cells. The present study shows that CRA significantly inhibits cell viability in a concentration- and time-dependent manner. Exposure to CRA induces sub-G1 cell cycle arrest and causes apoptotic death in A549 cells. CRA also triggers the activation of caspases and poly(ADP-ribose) polymerase, an effect antagonized by z-vad-fmk. In addition, exposure to CRA leads to a significant increase in the levels of reactive oxygen species (ROS) inA549 cells. Furthermore, exposure to the ROS scavenger N acetylcysteine (NAC)prevents CRA-induced apoptosis, suggesting a role for ROS in CRA-induced apoptosis. ROS are critical regulators of caspase-mediated apoptosis in A549 cells. These results indicate that CRA induces mitochondria-mediated and caspase-dependent apoptosis inA549 cells by altering anti-apoptotic proteins in a ROS-dependent manner.