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Amelioration of cyclophosphamide-induced hepatotoxicity by the root extract of Decalepis hamiltonii in mice

Zarei, Mahsa, Shivanandappa, T.
Food and chemical toxicology 2013 v.57 pp. 179-184
alanine, alanine transaminase, alkaline phosphatase, antioxidant activity, antioxidants, aspartate transaminase, blood serum, catalase, cyclophosphamide, drug therapy, gene expression regulation, genes, glutathione, glutathione peroxidase, glutathione transferase, glutathione-disulfide reductase, hepatotoxicity, lactate dehydrogenase, lipid peroxidation, liver, mice, oxidative stress, reactive oxygen species, roots, superoxide dismutase, toxicology
Hepatoprotective potential of the aqueous extract of the roots of Decalepis hamiltonii (DHA) against cyclophosphamide (CP)-induced oxidative stress has been investigated in mice. Administration of CP (25mg/kg b.w., i.p) for 10days induced hepatic damage as indicated by the serum marker enzymes aspartate and alanine transaminases (AST, ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH). Parallel to these changes CP induced oxidative stress in the liver as evident from the increased lipid peroxidation (LPO), reactive oxygen species (ROS), depletion of glutathione (GSH), and reduced activities of the antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST). Treatment with DHA (50 and 100mg/kg b.w., po) mitigated the CP-induced oxidative stress. Moreover, expression of genes for the antioxidant enzymes, were down-regulated by CP treatment which was reversed by DHA. Our study shows the DHA protected the liver from toxicity induced by CP and therefore, it could be serve as a safe medicinal supplement during cyclophosphamide chemotherapy.