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Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis

Author:
Riding, Matthew J., Trevisan, Júlio, Hirschmugl, Carol J., Jones, Kevin C., Semple, Kirk T., Martin, Francis L.
Source:
Environment international 2012 v.50 pp. 56-65
ISSN:
0160-4120
Subject:
biochemical compounds, eukaryotic cells, image analysis, multivariate analysis, nanomaterials, toxicity
Abstract:
Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics.
Agid:
868545