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Cytotoxic constituents from the leaves of Jerusalem artichoke (Helianthus tuberosus L.) and their structure–activity relationships

Yuan, Xiaoyan, Cheng, Mengchun, Gao, Mingzhe, Zhuo, Rongjie, Zhang, Liangxiao, Xiao, Hongbin
Phytochemistry letters 2013 v.6 no.1 pp. 21-25
Helianthus tuberosus, Jerusalem artichokes, anticarcinogenic activity, cytotoxicity, flavones, inhibitory concentration 50, leaves, neoplasm cells, nuclear magnetic resonance spectroscopy, sesquiterpenoid lactones, structure-activity relationships
Bioassay-directed phytochemical study of the Jerusalem artichoke (Helianthus tuberosus L.) leaves led to the isolation of a new sesquiterpene lactone of 3-Hydroxy-8β-tigloyloxy-1,10-dehydroariglovin (1), ten known sesquiterpene lactones (2–11) and two known flavones (12–13). Their chemical structures were elucidated on the basis of NMR (1D and 2D) and mass spectroscopic analysis. The cytotoxic activities of those compounds were subsequently tested against the MCF-7, A549 and HeLa cancer cells lines. The results indicated that sesquiterpene lactones 1–11 exhibited consistent cytotoxicity against all three cancer cell lines, while flavones 12 and 13 showed selective inhibitory activity against HeLa cell lines. Among them, compound 3 exhibited significant growth inhibitory activity against all three cell lines. The IC50 values of compound 3 against MCF-7, A549 and HeLa were 1.97±0.04, 7.79±0.44, 9.87±0.20μg/ml, respectively. In addition, some structure–activity relationships of these sesquiterpene lactones for cytotoxicity were explored and summarized in this study.