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Recovery of fat snook, Centropomus parallelus (Teleostei: Perciformes) after subchronic exposure to copper

Author:
Oss, Rodrigo Nunes, Baroni, Vinícius Dadalto, Duarte, Rafael Mendonça, Val, Adalberto Luis, Val, Vera Maria Fonseca Almeida, Gomes, Levy Carvalho
Source:
Comparative Biochemistry and Physiology, Part C 2013 v.157 pp. 306-309
ISSN:
1532-0456
Subject:
Centropomus parallelus, copper, fish, genotoxicity, gills, glutathione transferase, juveniles, muscle tissues, muscles, subchronic exposure
Abstract:
We studied the recovery of juvenile fat snook (Centropomus parallelus) after subchronic exposure to different concentrations of copper. Healthy juveniles (1.98g) were exposed to 25 or 50μg Cu/L for 30days (12 replicates with 5 fish in each one), and recovery was observed at 0, 4, 10, and 30days after exposure (3 replicates with 5 fish in each one). Copper genotoxicity in exposed individuals was observed using a micronucleus assay, and recovery was not observed even 30days post-exposure. Copper accumulation was observed in fish exposed to 25 or 50μg/L of copper in the gills (14.4 and 34.4μg/g, respectively) and muscle (5.7 and 5.5μg/g, respectively), and a return to normal copper levels (6.0μg/g for gills and 2.5μg/g for muscle) was observed 4 and 30days post-exposure in the gills and muscle tissues, respectively. Glutathione S-transferase (GST) was 80% inhibited in individuals exposed to copper and returned to normal levels for fish exposed to basal concentrations within 10days. Although copper accumulation in tissues dispersed 30days post-exposure, no recovery from genotoxicity was observed during this time. Thirty days was not enough to recover juvenile fat snook following subchronic exposure to copper.
Agid:
871944