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Molecular cloning and characterization of amh, dax1 and cyp19a1a genes and their response to 17α-methyltestosterone in Pengze crucian carp

Li, Meng, Wang, Lihong, Wang, Houpeng, Liang, Hongwei, Zheng, Yao, Qin, Fang, Liu, Shaozhen, Zhang, Yingying, Wang, Zaizhao
Comparative Biochemistry and Physiology, Part C 2013 v.157 pp. 372-381
Cyprinidae, adults, carp, complementary DNA, gene expression, genes, hatching, juveniles, liver, messenger RNA, molecular cloning, proteins, tissue distribution, tissues, triploidy
The proteins encoded by amh, dax1 and cyp19a1a play important roles in gonad differentiation. Their functions have been far less studied in teleosts. In this study, the full-length cDNAs of amh, dax1 and cyp19a1a were cloned and characterized in a triploid gynogenic fish, the Pengze crucian carp. Their expression profilings in juvenile development, adult tissues and juveniles exposed to 100ng/L 17α-methyltestosterone (MT) were investigated. Results showed that their putative proteins shared high identities to their counterparts in cyprinid fish species, respectively. The tissue distribution results indicated that amh and cyp19a1a were predominantly expressed in the ovary and dax1 was dominantly expressed in the liver. Gene profiling in the developmental stages showed that all the three target genes had a consistent highest expression at 48days post hatching (dph). The period of 48dph appeared to be a key time during the process of the gonad development of Pengze crucian carp. 100ng/L MT significantly increased the mRNA expression of amh at 2- and 4-week exposures and enhanced dax1 and cyp19a1a at 6-week exposure. The present study indicated that MT could influence the gonad development in Pengze crucian carp by disturbing sex-differentiation associated gene expression. Furthermore, the present study will be of great significance to broaden the understanding of molecular mechanisms of the physiological processes of reproduction in fish.