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A reverse-transcription, loop-mediated isothermal amplification assay for detection of bovine ephemeral fever virus in the blood of infected cattle

Author:
Zheng, Fuying, Lin, Guozhen, Zhou, Jizhang, Wang, Guanghua, Cao, Xiaoan, Gong, Xiaowei, Qiu, Changqing
Source:
Journal of virological methods 2011 v.171 no.1 pp. 306-309
ISSN:
0166-0934
Subject:
Bovine ephemeral fever virus, Rabies virus, blood, cattle, isolation techniques, restriction endonucleases, reverse transcriptase polymerase chain reaction, reverse transcription, reverse transcription loop-mediated isothermal amplification, serotypes, viruses
Abstract:
A novel reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) assay for the detection of bovine ephemeral fever virus (BEFV) was developed and evaluated in this study. The RT-LAMP assay exhibited higher sensitivity when compared with conventional reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation methods. The specificity of the assay was determined by digestion of the RT-LAMP products with restriction enzyme and detection of BEFV serogroup rabies virus (RV). Using RT-LAMP, RT-PCR and virus isolation methods, 36 blood samples were tested and the results indicated that RT-LAMP could detect early infection with BEFV. The RT-LAMP method is useful for the diagnosis of BEFV infection in blood samples.
Agid:
877023